Coding

Part:BBa_K5301022:Design

Designed by: Jiaxin Cao   Group: iGEM24_BNU-China   (2024-09-24)


SpyCatcher-spNW50-SpyTag achieving self-cyclization of MSP nanodisc.


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 565
    Illegal EcoRI site found at 1645
    Illegal SpeI site found at 517
    Illegal PstI site found at 733
    Illegal PstI site found at 1273
    Illegal PstI site found at 1306
    Illegal PstI site found at 1813
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 565
    Illegal EcoRI site found at 1645
    Illegal NheI site found at 133
    Illegal NheI site found at 2061
    Illegal SpeI site found at 517
    Illegal PstI site found at 733
    Illegal PstI site found at 1273
    Illegal PstI site found at 1306
    Illegal PstI site found at 1813
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 565
    Illegal EcoRI site found at 1645
    Illegal BglII site found at 993
    Illegal BglII site found at 1533
    Illegal BglII site found at 1701
    Illegal BamHI site found at 166
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 565
    Illegal EcoRI site found at 1645
    Illegal SpeI site found at 517
    Illegal PstI site found at 733
    Illegal PstI site found at 1273
    Illegal PstI site found at 1306
    Illegal PstI site found at 1813
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 565
    Illegal EcoRI site found at 1645
    Illegal SpeI site found at 517
    Illegal PstI site found at 733
    Illegal PstI site found at 1273
    Illegal PstI site found at 1306
    Illegal PstI site found at 1813
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This pathway expresses the SpyCatcher-NW50-SpyTag fusion protein under IPTG induction, achieving self-cyclization of NW50 to construct nanodiscs.


Source

NW50s are amphipathic proteins composed of a set of α-helixes derived from apolipoprotein A-1 (ApoA1).

References